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jacalin conjugated agarose beads  (Vector Laboratories)


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    Vector Laboratories jacalin conjugated agarose beads
    Jacalin Conjugated Agarose Beads, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 92/100, based on 31 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jacalin conjugated agarose beads/product/Vector Laboratories
    Average 92 stars, based on 31 article reviews
    jacalin conjugated agarose beads - by Bioz Stars, 2026-06
    92/100 stars

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    jacalin-conjugated agarose beads  (Millipore)
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    Analysis of O-linked carbohydrate on gO and gO complexes. Solubilized virions were incubated overnight <t>with</t> <t>jacalin-conjugated</t> agarose beads. After washing was done, bound proteins were eluted from the beads and analyzed by SDS-PAGE and immunoblotting with gO antibody. V, total solubilized virion proteins; A, proteins eluted from control streptavidin-agarose beads; J, proteins eluted from jacalin-agarose beads. (A) Prior to incubation with beads, virion proteins were boiled, reduced, and treated with PNGase to remove N-glycans. (B) Unreduced, fully glycosylated virion proteins were incubated with jacalin beads. Arrows indicate gH-gL-gO complexes, and arrowheads indicate previously unidentified forms of gO. Numbers beside panels show kilodaltons.
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    agarose conjugated jacalin beads  (Thermo Fisher)
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    Analysis of O-linked carbohydrate on gO and gO complexes. Solubilized virions were incubated overnight <t>with</t> <t>jacalin-conjugated</t> agarose beads. After washing was done, bound proteins were eluted from the beads and analyzed by SDS-PAGE and immunoblotting with gO antibody. V, total solubilized virion proteins; A, proteins eluted from control streptavidin-agarose beads; J, proteins eluted from jacalin-agarose beads. (A) Prior to incubation with beads, virion proteins were boiled, reduced, and treated with PNGase to remove N-glycans. (B) Unreduced, fully glycosylated virion proteins were incubated with jacalin beads. Arrows indicate gH-gL-gO complexes, and arrowheads indicate previously unidentified forms of gO. Numbers beside panels show kilodaltons.
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    Analysis of O-linked carbohydrate on gO and gO complexes. Solubilized virions were incubated overnight with jacalin-conjugated agarose beads. After washing was done, bound proteins were eluted from the beads and analyzed by SDS-PAGE and immunoblotting with gO antibody. V, total solubilized virion proteins; A, proteins eluted from control streptavidin-agarose beads; J, proteins eluted from jacalin-agarose beads. (A) Prior to incubation with beads, virion proteins were boiled, reduced, and treated with PNGase to remove N-glycans. (B) Unreduced, fully glycosylated virion proteins were incubated with jacalin beads. Arrows indicate gH-gL-gO complexes, and arrowheads indicate previously unidentified forms of gO. Numbers beside panels show kilodaltons.

    Journal:

    Article Title: Distinct Glycoprotein O Complexes Arise in a Post-Golgi Compartment of Cytomegalovirus-Infected Cells

    doi: 10.1128/JVI.76.6.2890-2898.2002

    Figure Lengend Snippet: Analysis of O-linked carbohydrate on gO and gO complexes. Solubilized virions were incubated overnight with jacalin-conjugated agarose beads. After washing was done, bound proteins were eluted from the beads and analyzed by SDS-PAGE and immunoblotting with gO antibody. V, total solubilized virion proteins; A, proteins eluted from control streptavidin-agarose beads; J, proteins eluted from jacalin-agarose beads. (A) Prior to incubation with beads, virion proteins were boiled, reduced, and treated with PNGase to remove N-glycans. (B) Unreduced, fully glycosylated virion proteins were incubated with jacalin beads. Arrows indicate gH-gL-gO complexes, and arrowheads indicate previously unidentified forms of gO. Numbers beside panels show kilodaltons.

    Article Snippet: Jacalin-conjugated agarose beads (Sigma) were equilibrated in radioimmunoprecipitation assay (RIPA) medium-0.5% bovine serum albumin (BSA; Sigma) prior to use.

    Techniques: Incubation, SDS Page, Western Blot